A display panel and a manufacturing method thereof and a display device using the same are provided. The display panel includes a display region and a non-display region. The display panel includes a substrate, a plurality of thin film transistors (TFTs) and a planarization layer sequentially stacked and at least one buffer unit disposed between the planarization layer and the substrate, wherein the buffer unit is located outside the TFT. The buffer unit is positioned in the display region and used for buffering stress of the display panel during bending.