A flexible organic light-emitting diode (OLED) display substrate and manufacturing method using the same are provided. The flexible OLED display substrate includes a display region, a non-display region, and a bending region connected to the display region and the non-display region. The bending region is provided with multiple liquid conductive wires spaced from each other. Each liquid conductive wire includes a conductive flow channel disposed in the bending region and a pair of metal wire portions connected to two ends of the conductive flow channel. A packaging layer is arranged on each conductive flow channel to store a liquid conductive material. The liquid conductive material is sealed in each conductive flow channel. Therefore, the liquid conductive wires can greatly improve the production yield of the bending region, and overcome a problem that a conventional bending region is prone to break during a bending process.