A battery holder capable of simplifying assembly procedure includes: case body, having at least three battery chambers; and a connection assembly, having at least one anode/cathode connecting unit, an anode output terminal, a cathode output terminal and an anode/cathode jumping unit, thereby allowing the at least three battery chambers to be in a serial connecting status; and characterized in that: an accommodation slot is formed between two of the at least three battery chambers which are arranged at outer sides, the anode/cathode jumping unit is formed through a metal wire being bent, and has a connection segment accommodated in the accommodation slot, one end of the connection segment has a cathode elastic contact point connected to the battery chamber arranged at the outer side, and another end thereof has an anode contact point connected to the other battery chamber arranged at the outer side.