Invention Grant
- Patent Title: Compositions and methods for identifying and sorting antigen-specific B cells
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Application No.: US16335891Application Date: 2017-09-22
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Publication No.: US11236153B2Publication Date: 2022-02-01
- Inventor: Arash Grakoui , Joseph Marcotrigiano
- Applicant: Emory University , Rutgers, The State University of New Jersey
- Applicant Address: US GA Atlanta; US NJ New Brunswick
- Assignee: Emory University,Rutgers, The State University of New Jersey
- Current Assignee: Emory University,Rutgers, The State University of New Jersey
- Current Assignee Address: US GA Atlanta; US NJ New Brunswick
- Agency: Emory Patent Group
- International Application: PCT/US2017/052882 WO 20170922
- International Announcement: WO2018/057843 WO 20180329
- Main IPC: A61K39/00
- IPC: A61K39/00 ; A61K39/21 ; C12N7/00 ; A61K39/12 ; C12N15/00 ; C07K16/10 ; C12N15/10 ; C12Q1/686
Abstract:
The present disclosure relates to constructs useful in expressing biotinylated monomers and tetramers produced from these monomers. Specifically, the disclosure provides a construct useful in expressing biotinylated antigen monomers, said construct comprising a viral protein of an ectodomain of Hepatitis C Virus (HCV) envelope glycoprotein E2 or Human Immunodeficiency Virus (HIV) gp140. Further disclosed are methods for production and use of these tetramers in identifying and isolating antigen specific B cells and cloning antibodies thereto.
Public/Granted literature
- US20200181242A1 Compositions and Methods for Identifying and Sorting Antigen-Specific B Cells Public/Granted day:2020-06-11
Information query
