Invention Grant
- Patent Title: Methods and compositions for producing stem cell derived dopaminergic cells for use in treatment of neurodegenerative diseases
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Application No.: US16414848Application Date: 2019-05-17
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Publication No.: US11236302B2Publication Date: 2022-02-01
- Inventor: Agnete Kirkeby , Malin Pernilla Parmar
- Applicant: BIOLAMINA AB
- Applicant Address: SE Sundbyberg
- Assignee: BIOLAMINA AB
- Current Assignee: BIOLAMINA AB
- Current Assignee Address: SE Sundbyberg
- Agent Jianjie Hu
- Main IPC: C12N5/0797
- IPC: C12N5/0797 ; C12N5/0793 ; A61K35/545 ; A61K35/30

Abstract:
The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 in cell culture medium containing a GSK3 inhibitor and a TGF-β inhibitor as well as timely administered fibroblast growth factor, desired neural cells are produced at far higher rates. Useful cell culture kits for producing such dopaminergic cells are also described herein, as are methods of using such cells for stem cell therapy.
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