Invention Grant
- Patent Title: Recombinant vector and method for producing reconstituted cytochrome P450 oxygenase-reductase fusion protein using the same
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Application No.: US16995168Application Date: 2020-08-17
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Publication No.: US11236309B2Publication Date: 2022-02-01
- Inventor: Geun Joong Kim , Chul Ho Yun , Dae Eun Cheong , Su Kyoung Yoo , Hye Ji Choi , Thien Kim Le
- Applicant: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
- Applicant Address: KR Gwangju
- Assignee: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
- Current Assignee: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
- Current Assignee Address: KR Gwangju
- Agency: Pleechae IP, LLC
- Priority: KR10-2020-0057020 20200513
- Main IPC: C12N9/02
- IPC: C12N9/02 ; C12N15/70 ; C07K14/32 ; C12P3/00

Abstract:
A recombinant vector according to an embodiment of the present invention may be used for producing a reconstituted cytochrome P450 oxygenase-reductase fusion protein. Oxygenase and reductase may be independently expressed in a host cell into which the recombinant vector is introduced, and then may be fused with a split intein so that it is possible to increase the heme content contained in the active site of the oxygenase and improve enzyme activity and stability.
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