Invention Grant
- Patent Title: T7 RNA polymerase variants
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Application No.: US16938793Application Date: 2020-07-24
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Publication No.: US11236311B2Publication Date: 2022-02-01
- Inventor: Mathew G. Miller , Chinping Chng , Oscar Alvizo , Melissa Ann Mayo , James Nicholas Riggins , Xiang Yi , Jonathan S. Penfield , Gjalt W. Huisman , Jared Davis , Yasushi Saotome
- Applicant: Codexis, Inc.
- Applicant Address: US CA Redwood City
- Assignee: Codexis, Inc.
- Current Assignee: Codexis, Inc.
- Current Assignee Address: US CA Redwood City
- Agency: Codexis, Inc.
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12N1/20 ; C12N15/00 ; C12P19/34
Abstract:
The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.
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