Invention Grant
- Patent Title: Method for producing dextran polymer-based, amplified nucleic acid aptamer nanoconstruct selectively capturing target molecule
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Application No.: US16346251Application Date: 2017-11-01
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Publication No.: US11236343B2Publication Date: 2022-02-01
- Inventor: Won Jong Kim , Jeong Hun Kim , Jihyun Lee , Byung Joo Lee
- Applicant: INSTITUTE FOR BASIC SCIENCE , POSTECH ACADEMY-INDUSTRY FOUNDATION
- Applicant Address: KR Daejeon; KR Pohang-si
- Assignee: INSTITUTE FOR BASIC SCIENCE,POSTECH ACADEMY-INDUSTRY FOUNDATION
- Current Assignee: INSTITUTE FOR BASIC SCIENCE,POSTECH ACADEMY-INDUSTRY FOUNDATION
- Current Assignee Address: KR Daejeon; KR Pohang-si
- Agency: Lex IP Meister, PLLC
- Priority: KR10-2016-0144400 20161101
- International Application: PCT/KR2017/012242 WO 20171101
- International Announcement: WO2018/084562 WO 20180511
- Main IPC: C12N15/115
- IPC: C12N15/115 ; A61K47/61 ; A61P27/02 ; A61P35/00 ; A61K31/713

Abstract:
The present invention relates to a method for producing dextran polymer-based amplified nucleic acid aptamer nanoconstructs which efficiently and selectively capture a specific target molecule, the method comprising linking a short nucleic acid sequence or a complementary sequence for formation of nanoconstructs and a nucleic acid aptamer sequence for capture of the specific target molecule to a dextran polymer by a chemical reaction, mixing the resulting polymer/nucleic acid substances with each other to form nanostructures, subjecting the nanostructures to rolling circle amplification, thereby forming a nucleic acid aptamer having a repeated structure. The dextran polymer-based amplified nucleic acid aptamer nanoconstructs have the effect of efficiently and selectively capturing a specific target molecule.
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