The present invention relates to a method for generating a biometric signature of a subject comprising: obtaining a plurality of sequential video frame images of a moving subject from a video segment; obtaining a portion of each frame comprising a surrounding of the moving subject; carrying out a transformation function to the frequency domain on one or more of said portions of the frames comprising a surrounding of a of the subject; and optionally saving the spectral characteristics of said transformation function in a repository. The present invention also relates to a system for carrying out said method.