Invention Grant
- Patent Title: Microscopic imaging method using a correction factor
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Application No.: US16445802Application Date: 2019-06-19
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Publication No.: US11238575B2Publication Date: 2022-02-01
- Inventor: Viktor Drescher , Nils Langholz
- Applicant: Carl Zeiss Microscopy GmbH
- Applicant Address: DE Jena
- Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee Address: DE Jena
- Agency: Duane Morris LLP
- Priority: DE102018210606.1 20180628
- Main IPC: G06T5/50
- IPC: G06T5/50 ; H04N5/225 ; H04N5/232 ; G02B21/00 ; G06T5/00 ; H04N5/32 ; G06T7/00

Abstract:
A microscopic imaging method, includes illuminating a specimen with illumination radiation and capturing detection radiation along a detection axis. The detection radiation is caused by the illumination radiation, at a first time as a wide-field signal and at a second time as a composite signal. The composite signal is formed by a superposition of a confocal image and a wide-field image; extracting the confocal image by subtracting the wide-field signal from the composite signal, wherein a correction factor is used. A current correction factor is ascertained for each executed imaging and/or for each imaged specimen (1) and the confocal image is extracted using the respective current correction factor.
Public/Granted literature
- US20200005442A1 MICROSCOPIC IMAGING METHOD USING A CORRECTION FACTOR Public/Granted day:2020-01-02
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