The embodiments of the present disclosure disclose a pixel circuit and a driving method thereof, a display substrate, and a display device, the present disclosure belongs to the field of displaying. The pixel circuit includes a gate line, a data line, a first charging sub-circuit, a second charging sub-circuit and a display sub-circuit; the first charging sub-circuit is configured to be controllable to output a data signal from the data line to a charging node and to store the data signal from the data line; and the second charging sub-circuit is respectively connected to the charging node, the gate line and the display sub-circuit, and is configured to be controllable to output a data signal from the charging node to the display sub-circuit.