A charge release circuit, a display substrate, a display device and a charge release method thereof are provided. The charge release circuit including: a controller, a charge release sub-circuit and a first conductor, wherein the charge release sub-circuit is respectively connected with the controller, the first conductor and a second conductor in an active area of an array substrate, and the charge release sub-circuit is configured to conduct the first conductor and the second conductor under a control of the controller, so as to allow charges on the second conductor to move to the first conductor. The charge release circuit can solve the problem that the display panel in the black-screen state displays bright spots so as to reduce the number of bright spots on the display panel in the black-screen state.