A display substrate having a display area and a peripheral area is provided. The display substrate in the peripheral area includes a flexible base substrate; a first insulating layer on the flexible base substrate; a first signal line layer including a plurality of first signal lines on a side of the first insulating layer away from the flexible base substrate; and a second insulating layer on a side of the first signal line layer away from the flexible base substrate. The display substrate has a plurality of first vias extending through a respective one of the plurality of first signal lines, exposing a first surface of the first insulating layer; and the second insulating layer extends through the plurality of first vias to be in direct contact with the first surface of the first insulating layer.