A display substrate includes a base substrate, a color filter layer, a black matrix and a passivation layer. The color filter layer, the black matrix and the passivation layer are arranged on the base substrate, the color filter layer includes: a plurality of color filter units; a plurality of white filter unit formed by a blank area; a black matrix disposed between the color filter unit and the white filter unit; and a pad layer arranged in the blank area, an orthogonality projection of the pad layer on the base substrate being located in an orthogonality projection of the blank area on the base substrate, and smaller than the orthogonality projection of the blank area on the base substrate.