The present disclosure relates to a pressure sensing module, a touch panel and a method of detecting a two-point touch pressure of a touch panel. The pressure sensing module includes a substrate and a sensing layer formed over the substrate. The sensing layer includes a plurality of pressure sensing units. The pressure sensing units are axially symmetrically disposed along a symmetry axis of the substrate to form a first pressure sensing region and a second pressure sensing region. The pressure sensing unit includes four resistors having a same resistance. The four resistors are configured as a Wheatstone bridge, in which pattern shapes of two of the resistors have a same extending direction and are not adjacent. The touch panel includes the pressure sensing module described above. The method of detecting the two-point touch pressure of the touch panel is used to detect the two-point touch pressure on the touch panel.