The present invention is directed to a method for segmentation and tracking of objects recorded in a grayscale video stream and three-dimensional image slices obtained from various devices, including microscopes, video cameras, medical apparatus, and remote sensing devices, using an approach to correct background, segment images using an improved approach, track a segmented area by evaluating surrounding information of the area, analyze data, and create archives of video streams or three-dimensional image slices, segmented areas and the results of tracking.