A driving method applicable to a display device, in which the display device includes multiple pixel circuits, and the method includes: adjusting multiple control signals according to a first display data such that the pixel circuits generate a first frame; receiving a second display data generated after the display data; and adjusting the control signals according to a time duration of the first frame such that the pixel circuits generate a second frame, where brightness for each of the pixel circuits is proportional to a duty ratio of one of the corresponding control signals, and an increment for the duty ratio of one of the corresponding control signals in the second frame is negatively correlated to a difference between a ratio of a preset time period to the time duration of the first frame and a ratio of the preset time period to the time duration of the second frame.