A device and a method for breeding blueberry-specific mycorrhizal fungi. The device includes a container, where a bottom of the container is laid with perlite to form a perlite layer; an absorbent cotton is arranged on the perlite layer; a hole for planting is provided in the absorbent cotton and blueberry tissue culture seedlings or moss seedlings are placed in the hole; and a spore transfer solution is provided around the blueberry tissue culture seedlings or moss seedlings. When used as a bacterial fertilizer for inoculation in the field, the pieces of absorbent cotton are buried directly around the blueberry roots; while used for research, one of the pieces of the absorbent cotton is washed with sterile water and filtered with a filter paper to obtain mycorrhizal fungi spores.