Invention Grant
- Patent Title: Methods and compositions for influenza a virus subtyping
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Application No.: US16313330Application Date: 2017-06-23
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Publication No.: US11248272B2Publication Date: 2022-02-15
- Inventor: Genyan Yang , Charles Todd Davis
- Applicant: The United States of America, as represented by the Secretary, Dept. of Health and Human Services
- Applicant Address: US MD Bethesda
- Assignee: The United States of America, as represented by the Secretary, Dept. of Health and Human Services
- Current Assignee: The United States of America, as represented by the Secretary, Dept. of Health and Human Services
- Current Assignee Address: US MD Bethesda
- Agency: Klarquist Sparkman, LLP
- International Application: PCT/US2017/039018 WO 20170623
- International Announcement: WO2018/005284 WO 20180104
- Main IPC: C12Q1/70
- IPC: C12Q1/70 ; C12Q1/6816 ; C12Q1/6806 ; C12Q1/686

Abstract:
Disclosed herein are methods for detecting presence of a target nucleic acid (such as an influenza virus nucleic acid) in a sample. In some embodiments, the methods include contacting the sample with a first probe capable of hybridizing to the target nucleic acid and a second probe capable of hybridizing to the target nucleic acid, contacting the resulting complex with one or more gap filling reagents, thereby producing a gap-filled target nucleic acid, isolating and amplifying the gap-filled target nucleic acid. The amplified gap-filled target nucleic acid covalently linked to the substrate is then detected, for example with a detectably labeled probe. Also disclosed herein are probes capable of hybridizing to influenza virus nucleic acids. The disclosure also includes kits for detecting and/or discriminating influenza virus nucleic acids in a sample. In some examples, the kits include two or more of the disclosed probes.
Public/Granted literature
- US20190153549A1 METHODS AND COMPOSITIONS FOR INFLUENZA A VIRUS SUBTYPING Public/Granted day:2019-05-23
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