Invention Grant
- Patent Title: Method for producing cellulase and apparatus for said method
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Application No.: US14359122Application Date: 2012-11-21
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Publication No.: US11253818B2Publication Date: 2022-02-22
- Inventor: Hiroyuki Kurihara , Katsushige Yamada
- Applicant: Toray Industries, Inc.
- Applicant Address: JP Tokyo-to
- Assignee: Toray Industries, Inc.
- Current Assignee: Toray Industries, Inc.
- Current Assignee Address: JP Tokyo-to
- Agency: DLA Piper LLP (US)
- Priority: JPJP2011-253706 20111121
- International Application: PCT/JP2012/080123 WO 20121121
- International Announcement: WO2013/077341 WO 20130530
- Main IPC: B01D61/18
- IPC: B01D61/18 ; C12M1/00 ; C12P21/00 ; B01D61/16 ; B01D61/14 ; C12N9/42

Abstract:
A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.
Public/Granted literature
- US20140295525A1 METHOD FOR PRODUCING CELLULASE AND APPARATUS FOR SAID METHOD Public/Granted day:2014-10-02
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