Invention Grant
- Patent Title: Method, substrate and kit for one-pot one-step assembly of DNA molecules
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Application No.: US16071822Application Date: 2017-02-01
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Publication No.: US11254929B2Publication Date: 2022-02-22
- Inventor: Sylvestre Marillonnet
- Applicant: Leibnitz-Institut fuer Pflanzenbiochemie (IPB)
- Applicant Address: DE Halle
- Assignee: Leibnitz-Institut fuer Pflanzenbiochemie (IPB)
- Current Assignee: Leibnitz-Institut fuer Pflanzenbiochemie (IPB)
- Current Assignee Address: DE Halle
- Agency: Patent Central LLC
- Agent Stephan A. Pendorf
- Priority: DE102016101948.8 20160204
- International Application: PCT/DE2017/200014 WO 20170201
- International Announcement: WO2017/133738 WO 20170810
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12N15/10 ; C12Q1/6806 ; C12N15/66
Abstract:
A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule, and a substrate and a kit for this purpose. A simple and cost-effective assembly method for DNA molecules. A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule is provided, wherein the two or more DNA molecules to be assembled are brought together in dry form with a suitable reaction medium on at least one substrate present in a reaction vessel.
Public/Granted literature
- US20190032040A1 METHOD, SUBSTRATE AND KIT FOR ONE-POT ONE-STEP ASSEMBLY OF DNA MOLECULES Public/Granted day:2019-01-31
Information query
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