Invention Grant
- Patent Title: RNA fixation and detection in clarity-based hydrogel tissue
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Application No.: US16075895Application Date: 2017-02-09
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Publication No.: US11254974B2Publication Date: 2022-02-22
- Inventor: Karl A. Deisseroth , Emily L. Sylwestrak , Priya Rajasethupathy , Matthew Wright
- Applicant: The Board of Trustees of the Leland Stanford Junior University
- Applicant Address: US CA Stanford
- Assignee: The Board of Trustees of the Leland Stanford Junior University
- Current Assignee: The Board of Trustees of the Leland Stanford Junior University
- Current Assignee Address: US CA Stanford
- Agency: Bozicevic, Field & Francis LLP
- Agent Edward J. Baba
- International Application: PCT/US2017/017251 WO 20170209
- International Announcement: WO2017/139501 WO 20170817
- Main IPC: C12Q1/6841
- IPC: C12Q1/6841 ; C12Q1/6806 ; G02B21/06 ; C12Q1/6837 ; G01N1/30 ; C12Q1/68 ; G02B21/00
Abstract:
Methods, kits, and systems for fixation of RNA permitting its detection in intact tissue, such as, large volume of mammalian tissue are disclosed. The methods, kits, and systems utilize carbodiimide-based chemistry to stably retain RNAs in tissue clarified using CLARITY. Also provided herein are methods, kits, and systems for detection of RNAs in clarified tissue.
Public/Granted literature
- US20190119735A1 RNA Fixation and Detection in CLARITY-based Hydrogel Tissue Public/Granted day:2019-04-25
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