Invention Grant
- Patent Title: Chimeric gene constructs for generation of fluorescent transgenic ornamental fish
-
Application No.: US15674798Application Date: 2017-08-11
-
Publication No.: US11259509B2Publication Date: 2022-03-01
- Inventor: Zhiyuan Gong , Jiangyan He , Bensheng Ju , Toong Jin Lam , Yanfei Xu , Tie Yan
- Applicant: National University of Singapore
- Applicant Address: SG Singapore
- Assignee: National University of Singapore
- Current Assignee: National University of Singapore
- Current Assignee Address: SG Singapore
- Agency: Merchant & Gould P.C.
- Priority: SG9900811-2 19990218,SG9903367-2 19990714
- Main IPC: A01K67/027
- IPC: A01K67/027 ; C07K14/435 ; C12N15/85 ; G01N33/74

Abstract:
Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
Public/Granted literature
- US20180064074A1 CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH Public/Granted day:2018-03-08
Information query