Invention Grant
- Patent Title: Methods of making guide RNA
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Application No.: US15760372Application Date: 2016-09-16
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Publication No.: US11261439B2Publication Date: 2022-03-01
- Inventor: Alejandro Chavez , Johnny Hao Hu
- Applicant: President and Fellows of Harvard College
- Applicant Address: US MA Cambridge
- Assignee: President and Fellows of Harvard College
- Current Assignee: President and Fellows of Harvard College
- Current Assignee Address: US MA Cambridge
- Agency: Banner & Witcoff, Ltd.
- International Application: PCT/US2016/052201 WO 20160916
- International Announcement: WO2017/049129 WO 20170323
- Main IPC: C12N15/10
- IPC: C12N15/10
Abstract:
A nucleic acid construct is provided that encodes two or more or a plurality of spacer sequences separated by restriction endonuclease recognition site. A plurality of such nucleic acid sequences are provided as a library for making guide RNAs for use with CRISPR/Cas systems.
Public/Granted literature
- US20200283758A1 Methods of Making Guide RNA Public/Granted day:2020-09-10
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