- Patent Title: Peptide sequence of a guide protein for the production of a peptide of interest, an expression vector, a host cell, and a method for the production of a peptide of interest
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Application No.: US16474805Application Date: 2017-12-28
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Publication No.: US11261472B2Publication Date: 2022-03-01
- Inventor: Juan Alfonso Asenjo De Leuze , Barbara Anne Andrews Farrow , Vida Rodriguez Gallardo
- Applicant: UNIVERSIDAD DE CHILE
- Applicant Address: CL Santiago
- Assignee: UNIVERSIDAD DE CHILE
- Current Assignee: UNIVERSIDAD DE CHILE
- Current Assignee Address: CL Santiago
- Agency: Lucas & Mercanti, LLP
- Priority: CL201603427 20161230
- International Application: PCT/IB2017/058480 WO 20171228
- International Announcement: WO2018/122777 WO 20180705
- Main IPC: C12P21/02
- IPC: C12P21/02 ; C07K14/46 ; C12N15/62 ; C12N15/67

Abstract:
A peptide sequence of a guide protein for the production of a peptide of interest; a peptide sequence that has a similarity of at least 90% to SEQ. ID. NO 1; a nucleotide sequence encoding the guide protein; an expression vector comprising the nucleotide sequence; a host cell that expresses a fusion protein comprising a peptide of interest; a method of production of a peptide of interest, comprising the steps of A) constructing an expression vector; B) inserting the expression vector into a host cell; C) expressing the fusion protein, culturing the host cell in a culture medium; D) recovering the accumulated fusion protein in the host cell; E) cleaving the fusion protein; F) purifying the peptide of interest.
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