A specimen for analyzing the shape of an antistatic antifouling layer and a method for preparing the same are provided. The specimen includes Pt coated on an antistatic antifouling layer comprising a conductive polymer formed on a polymer substrate, so that a contrast difference between the polymer substrate layer and the antistatic antifouling layer is caused by the diffusion of Pt by means of the conductive polymer and the dyeing effect of the antifouling layer. Accordingly, it is possible to clearly distinguish the shape of the antistatic antifouling layer formed on the polymer substrate by using TEM.