The present disclosure provides a display substrate including: a first flexible substrate; a second flexible substrate; and a bonding layer between the first flexible substrate and the second flexible substrate and bonding the first and second flexible substrates to each other; wherein the second flexible substrate includes a plurality of first support parts and a second support part spacing the plurality of first support parts apart from each other; a side of each first support part distal to the first flexible substrate is provided thereon with a display unit; the second support part includes a first part and a second part other than the first part, and a signal line configured to connect display units is disposed on a side of the first part distal to the first flexible substrate; and a thickness of the second part is smaller than a thickness of the first support part.