An operating method and a non-volatile memory device are provided. The non-volatile memory device includes a memory array including a plurality of memory cells. The operating method includes applying a first program voltage signal to selected word lines connected to selected memory cells during a first program period and measuring a first threshold voltage, applying a second program voltage signal to the selected word lines during a second program period and measuring a second threshold voltage, applying a test bit line voltage signal to selected bit lines and applying a third program voltage signal to the selected word lines during a third program period and measuring a third threshold voltage and determining the enhanced bit line voltage by comparing a difference between the third threshold voltage and the second threshold voltage with a difference between the second threshold voltage and the first threshold voltage.