A pixel arrangement structure are provided, which includes a plurality of repeating units. Each of the plurality of repeating units includes two first sub-pixels, one second sub-pixel, and one third sub-pixel. Each of the first sub-pixel, the second sub-pixel and the third sub-pixel includes a pixel defining layer, the pixel defining layer includes a pixel defining layer opening to define an effective light emitting region of each sub-pixel; a plurality of first sub-pixels are arranged along the second direction to form a plurality of first sub-pixel groups, orthographic projections of pixel defining layer openings of the first sub-pixels in adjacent ones of the plurality of first sub-pixel groups on a straight line parallel to the second direction do not overlap each other.