Provided is a virtual road surface implementation-type bicycle simulator. The virtual road surface implementation-type bicycle simulator according to the present disclosure includes a rear wheel support portion that supports a rear wheel of a mounted bicycle and rotates together with rotation of the rear wheel; and a road surface implementation portion including a plurality of uneven portions that perform a protrusion operation at various heights on an outer peripheral surface of the rear wheel support portion in contact with the rear wheel, thereby providing a rider riding the bicycle with various road surface states which are virtually set.