Invention Grant
- Patent Title: N-terminal fusion partner for producing recombinant polypeptide, and method for producing recombinant polypeptide using same
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Application No.: US16963066Application Date: 2019-01-18
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Publication No.: US11267863B2Publication Date: 2022-03-08
- Inventor: Sung Gun Kim
- Applicant: PEPGENE INC.
- Applicant Address: KR Chungcheongbuk-do
- Assignee: PEPGENE INC.
- Current Assignee: PEPGENE INC.
- Current Assignee Address: KR Chungcheongbuk-do
- Agency: Sughrue Mion, PLLC
- Priority: KR10-2018-0006875 20180119,KR10-2018-0018232 20180214,KR10-2018-0018255 20180214,KR10-2018-0018278 20180214
- International Application: PCT/KR2019/000782 WO 20190118
- International Announcement: WO2019/143193 WO 20190725
- Main IPC: C07K14/635
- IPC: C07K14/635 ; C07K14/58 ; C07K14/605 ; C07K7/06 ; C07K7/08 ; C07K14/47

Abstract:
Disclosed are a novel N-terminal fusion partner, a fusion polypeptide including the fusion partner and a target polypeptide, and a method for producing a target polypeptide using the same. The novel fusion partner can enhance the yield of a target polypeptide (recombinant polypeptide) compared to the conventional fusion partners. Using the novel fusion partner is particularly beneficial in producing a target polypeptide having a relatively low molecular weight and an easily degradable amino terminus based on genetic recombination technologies. Further, the novel fusion polypeptide including the fusion partner can be expressed as inclusion bodies in a host cell and protected against proteases or the like in a host cell, which makes the target polypeptide produced stably. Therefore, in comparison to the conventional fusion partners, the novel fusion partner can be used to provide a method for producing a recombinant peptide with improved stability and yield.
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