Invention Grant
- Patent Title: Afucosylated protein, cell expressing said protein and associated methods
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Application No.: US15573702Application Date: 2016-05-13
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Publication No.: US11267899B2Publication Date: 2022-03-08
- Inventor: Sohang Chatterjee , Kavitha Iyer Rodrigues , Maloy Ghosh , Sunit Maity , Divya Unnikrishnan , Jahnabi Hazarika , Yogendra Manjunath Bangalore Muniraju , Sathyabalan Murugesan , Pavithra Mukunda , Bhargav Prasad , Veeresha Kamanagowda , Sanghamitra Bhattacharjee , Pravin Kumar Dakshinamurthy , Vivek Halan , Sankaranarayanan Srinivasan , Anuradha Hora , Bairavabalakumar Natarajan , Karthika Nair , Aswini Thanigaivel , Amol Maliwalave , Bharath Ravindra Shenoy , Sahana Bhima Rao , Subhra Prakash Chakrabarty , Ashvini Kumar Dubey , Amir Khan , Ankurina Sharma
- Applicant: Zumutor Biologies, Inc.
- Applicant Address: US MA Woburn
- Assignee: Zumutor Biologies, Inc.
- Current Assignee: Zumutor Biologies, Inc.
- Current Assignee Address: US MA Woburn
- Agency: Fish & Richardson P.C.
- Priority: IN194/CHE/2015 20150513
- International Application: PCT/IB2016/052774 WO 20160513
- International Announcement: WO2016/181357 WO 20161117
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C07K16/32 ; C12N15/113 ; C07K16/06 ; C12N9/22 ; C12N15/90
Abstract:
The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.
Public/Granted literature
- US20180171028A1 AFUCOSYLATED PROTEIN, CELL EXPRESSING SAID PROTEIN AND ASSOCIATED METHODS Public/Granted day:2018-06-21
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