- Patent Title: Methods for continuous sorting of cells based on molecular adhesion
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Application No.: US16348520Application Date: 2017-11-08
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Publication No.: US11268892B2Publication Date: 2022-03-08
- Inventor: Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
- Applicant: Georgia Tech Research Corporation
- Applicant Address: US GA Atlanta
- Assignee: Georgia Tech Research Corporation
- Current Assignee: Georgia Tech Research Corporation
- Current Assignee Address: US GA Atlanta
- Agency: Troutman Pepper Hamilton Sanders LLP
- Agent Ryan A. Schneider
- International Application: PCT/US2017/060662 WO 20171108
- International Announcement: WO2018/089512 WO 20180517
- Main IPC: B01L3/00
- IPC: B01L3/00 ; G01N15/10 ; G01N1/34 ; G01N33/50

Abstract:
Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.
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