A display panel, a manufacturing method thereof, and a display device are provided. The display panel includes: a first base substrate including a packaging region; a plurality of support portions located in the packaging region, a first interval being provided between adjacent support portions; a plurality of conductive lines located at a side of the plurality of support portions away from the first base substrate; a packaging layer located at a side of the plurality of conductive lines away from the first base substrate; and a second base substrate arranged opposite to the first base substrate and bonded with the first base substrate by the packaging layer.