Invention Grant
- Patent Title: Enhanced ligation in sequencing library preparation
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Application No.: US15942214Application Date: 2018-03-30
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Publication No.: US11274344B2Publication Date: 2022-03-15
- Inventor: Craig Betts , Byoungsok Jung
- Applicant: GRAIL, Inc.
- Applicant Address: US CA Menlo Park
- Assignee: GRAIL, Inc.
- Current Assignee: GRAIL, Inc.
- Current Assignee Address: US CA Menlo Park
- Agency: Haynes and Boone LLP
- Agent Benjamin C. Pelletier
- Main IPC: C12Q1/6874
- IPC: C12Q1/6874 ; C12N15/10 ; C12Q1/6806 ; C12N15/66 ; C12Q1/6869
Abstract:
Methods for preparing a sequencing library from a DNA-containing test sample are provided. In some embodiments, the methods involve rescuing a partially ligated DNA fragment to enhance library preparation conversion efficiencies. In some embodiments, the methods involve improving recovery of duplex sequence information from double-stranded DNA.
Public/Granted literature
- US20180291445A1 ENHANCED LIGATION IN SEQUENCING LIBRARY PREPARATION Public/Granted day:2018-10-11
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