A radionuclide generation system including a tube system configured to permit insertion and removal of irradiation targets into an instrumentation finger of a nuclear reactor, and an irradiation target drive system configured to insert the irradiation targets into the instrumentation finger and to remove the irradiation targets from the instrumentation finger. The radionuclide generation system further includes an instrumentation and control unit which is linked to an online core monitoring system and being configured to calculate optimal irradiation locations for the irradiation targets based on the actual state of the reactor as provided by the online core monitoring system.