Invention Grant
- Patent Title: Glucoamylase variants and polynucleotides encoding same
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Application No.: US16603639Application Date: 2018-04-10
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Publication No.: US11279921B2Publication Date: 2022-03-22
- Inventor: Tomoko Namoto , Noriko Tsutsumi , Keiichi Ayabe , Zhengfang Kang
- Applicant: NOVOZYMES A/S
- Applicant Address: DK Bagsvaerd
- Assignee: NOVOZYMES A/S
- Current Assignee: NOVOZYMES A/S
- Current Assignee Address: DK Bagsvaerd
- Agent David Fazzolare
- International Application: PCT/US2018/026815 WO 20180410
- International Announcement: WO2018/191215 WO 20181018
- Main IPC: C12N9/34
- IPC: C12N9/34 ; C12P19/02 ; C12P19/14
Abstract:
The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guy-coamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K, R, S(G)546P, T(V)549W, N503R, N539R+I541Y+T543V+A545S+S546GCGV+G547S+S548T+T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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