Invention Grant
- Patent Title: Iterative genome editing in microbes
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Application No.: US17332503Application Date: 2021-05-27
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Publication No.: US11279940B2Publication Date: 2022-03-22
- Inventor: Stephen Blaskowski , Sara da Luz Areosa Cleto , Cameron Coates , Aaron Miller , Sharon Nademanee , Melissa Netwal , Kedar Patel , Shawn Szyjka , Philip Weyman , Solomon Henry Stonebloom , Colin Scott Maxwell , Elizabeth Lauren Meier
- Applicant: Zymergen Inc.
- Applicant Address: US CA Emeryville
- Assignee: Zymergen Inc.
- Current Assignee: Zymergen Inc.
- Current Assignee Address: US CA Emeryville
- Agency: Cooley LLP
- Main IPC: C12N15/70
- IPC: C12N15/70 ; C12N9/22 ; C12N15/113 ; C12N15/77 ; C12N15/80 ; C12N15/81
Abstract:
The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.
Public/Granted literature
- US20210292774A1 ITERATIVE GENOME EDITING IN MICROBES Public/Granted day:2021-09-23
Information query
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