Isolated cDNA sequences encoding for highly conserved domains of plant viral pathogen genomes. The cDNA sequences are selected from the group consisting of those listed in Table I (SEQ ID NOs: 1-68). cDNA fusion constructs or chimeric transgene constructs comprising at least two different cDNA sequences selected from the list in Table I, and more specifically cDNA corresponding to at least two different viruses is described, along with methods of creating transgenic plants with broad-spectrum, durable resistance to multiple viral pathogens using these cDNA sequences.