A putter holding assembly for storing a putter golf club on a golf cart includes a tube for accommodating a handle of a putter golf club having a head of the putter golf club being exposed for gripping. A receiver is provided and the receiver is coupled to the tube. The receiver insertably receives the handle of the putter golf club when the handle of the putter golf club is inserted into the tube for storage. A pair of brackets is each positionable around the tube. Each of the brackets engages a structure on a golf cart for retaining the tube on the golf cart. A support is movably coupled to the tube and a towel is coupled to the support such that the towel is available to a golfer.