A one-way valve (10) comprises: a valve housing (100) having a first end portion, a second end portion, a valve housing channel (110), and a stopper projection (108) provided within the valve housing channel (110); a valve body (200) having a first end portion, a second end portion, and a valve body channel (202) in fluid communication with the valve housing channel (110), the second end portion of the valve body (200) being snap-fit to the first end portion of the valve housing (100); and a sealing plug (300) slidably disposed within the valve body channel (202), wherein an inner diameter of the valve housing channel (110) is greater than an inner diameter of the valve body channel (202) and an outer diameter of the sealing plug (300), and a distance between the stopper projection (108) and the second end portion of the valve body (200) is greater than a longitudinal length of the sealing plug (300). A tire repair glue bottle (1) having a one-way valve comprises: a bottle body (20) containing glue; an air intake device (40) attached to the bottle body (20); a bottle lid (50) attached to the bottle body (20); a glue tube (30) provided within the bottle body (20) and having one end connected to the bottle lid (50); and an one-way valve (10), wherein the second end portion of the valve housing (100) of the one-way valve (10) is connected to the other end of the glue tube (30).