There is provided a muffler structure of a saddle-type vehicle. The muffler structure is disposed at a downstream side of an exhaust pipe extending from an exhaust port of a cylinder head. A pipe is connected to the exhaust pipe and inserted into a muffler main body. The pipe is provided with an expansion chamber having an inner diameter that is larger than an inner diameter of the exhaust pipe. At least a part of the expansion chamber is disposed in the muffler main body, and a plurality of through holes are formed on an outer peripheral surface of the expansion chamber disposed in the muffler main body.