The present disclosure illustrates a driving method of a display panel and a driver device thereof. The method comprises steps of: applying drive voltages with opposite polarities to drive the positional corresponding sub-pixels of the two adjacent rows of pixel units, wherein the voltages with opposite polarities comprise voltage with positive polarity and voltage with negative polarity; applying voltages with positive polarity to a part of the positional corresponding sub-pixels of the two adjacent rows of pixel units; applying voltage with negative polarity to the other part of the positional corresponding sub-pixels of the two adjacent rows of pixel units; applying two drive voltages with opposite polarities to drive sub-pixels of the same pixel unit; applying drive voltages with at least two voltage levels to drive sub-pixels of the same pixel unit, and separating the plurality of sub-pixels according to the drive voltages with voltage levels.