A culture container includes: a culture container body; a partition plate disposed in the vicinity of a bottom of the culture container body to face the bottom so that a culture region is formed as a culture region between the partition plate and the bottom; and a sample inlet/outlet provided in the culture container body, the culture region and the sample inlet/outlet communicating with each other via an opening formed adjacent to one end of the partition plate. With the culture container and a culture method using this container, a large amount of adipocytes can be cultured at one time with easy handling. Dedifferentiation of a large amount of adipocytes and removal of cells having failed to dedifferentiate can be efficiently accomplished.