- Patent Title: Method for inducing differentiation of alveolar epithelial cells
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Application No.: US15555183Application Date: 2016-03-02
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Publication No.: US11299712B2Publication Date: 2022-04-12
- Inventor: Shimpei Gotoh , Yuki Yamamoto , Satoshi Konishi , Michiaki Mishima
- Applicant: KYOTO UNIVERSITY
- Applicant Address: JP Kyoto
- Assignee: KYOTO UNIVERSITY
- Current Assignee: KYOTO UNIVERSITY
- Current Assignee Address: JP Kyoto
- Agency: Lucas & Mercanti, LLP
- Priority: JPJP2015-045298 20150306
- International Application: PCT/JP2016/057254 WO 20160302
- International Announcement: WO2016/143803 WO 20160915
- Main IPC: C12N5/071
- IPC: C12N5/071

Abstract:
This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3β inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.
Public/Granted literature
- US20180051256A1 METHOD FOR INDUCING DIFFERENTIATION OF ALVEOLAR EPITHELIAL CELLS Public/Granted day:2018-02-22
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