Provided are a display panel, a display device and a method for preparing a display panel. The display panel includes an opening region, a transition region, and a display region. The display region at least partially surrounds the opening region, and the transition region is disposed between the display region and the opening region. The transition region of the display panel is further provided with at least one wall structure, and the at least one wall structure surrounds the opening region. The display panel further includes a substrate and a sub-pixel array disposed on the substrate and disposed in the display region, a first organic encapsulation layer, a second organic encapsulation layer, and a barrier structure. The first organic encapsulation layer is disposed on a side of the at least one wall structure facing the display region.