A display substrate, a method for manufacturing the same and a display device are provided. The method for manufacturing the display substrate includes: forming a control structure layer on a base substrate; forming a first planarization layer on the control structure layer; forming a support structure on the first planarization layer, a material of the support structure includes a negative photosensitive polymer; forming a protection layer enveloping the support structure on the first planarization layer formed with the support structure; forming a second planarization layer on the protection layer; and forming a first electrode on the second planarization layer, the first electrode is electrically connected to the control structure layer.