Invention Grant
- Patent Title: Biotinylated and oxidized LDL receptor and advanced glycation end product receptor produced using genetically engineered silkworm
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Application No.: US15515998Application Date: 2015-10-01
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Publication No.: US11311002B2Publication Date: 2022-04-26
- Inventor: Sachiko Machida , Miyuki Kuramochi , Mayumi Kameyama , Mari Yamamoto , Toshiro Kobori , Hideki Sezutsu , Ken-ichiro Tatematsu
- Applicant: National Agriculture and Food Research Organization
- Applicant Address: JP Tsukuba
- Assignee: National Agriculture and Food Research Organization
- Current Assignee: National Agriculture and Food Research Organization
- Current Assignee Address: JP Tsukuba
- Agency: Seed IP Law Group LLP
- Priority: JPJP2014-203282 20141001,JPJP2015-100546 20150515,JPJP2015-100547 20150515
- International Application: PCT/JP2015/005017 WO 20151001
- International Announcement: WO2016/051808 WO 20160407
- Main IPC: A01K67/027
- IPC: A01K67/027 ; C07K16/18 ; C07K14/705 ; A01K67/033 ; C07K16/00 ; C07K14/47 ; C12N9/00 ; G01N33/92
Abstract:
The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.
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