Invention Grant
- Patent Title: Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
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Application No.: US15105849Application Date: 2014-12-18
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Publication No.: US11318479B2Publication Date: 2022-05-03
- Inventor: Kevin T. Chapman , Eric D. Hobbs , Steven W. Short , Mark P. White , Daniele Malleo
- Applicant: Berkeley Lights, Inc.
- Applicant Address: US CA Emeryville
- Assignee: Berkeley Lights, Inc.
- Current Assignee: Berkeley Lights, Inc.
- Current Assignee Address: US CA Emeryville
- Agency: Barnes & Thornburg LLP
- Agent Kenneth E. Horton
- International Application: PCT/US2014/071323 WO 20141218
- International Announcement: WO2015/095623 WO 20150625
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; B03C5/00 ; B03C5/02 ; B03C11/00 ; B01L3/00

Abstract:
Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
Public/Granted literature
- US20170021366A1 CAPTURING SPECIFIC NUCLEIC ACID MATERIALS FROM INDIVIDUAL BIOLOGICAL CELLS IN A MICRO-FLUIDIC DEVICE Public/Granted day:2017-01-26
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