Invention Grant
- Patent Title: Method of amplifying circular DNA
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Application No.: US16371766Application Date: 2019-04-01
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Publication No.: US11319579B2Publication Date: 2022-05-03
- Inventor: Masayuki Su'etsugu , Hiroko Kobayashi
- Applicant: OriCiro Genomics, Inc.
- Applicant Address: JP Tokyo
- Assignee: OriCiro Genomics, Inc.
- Current Assignee: OriCiro Genomics, Inc.
- Current Assignee Address: JP Tokyo
- Agency: Mintz Levin Cohn Ferris Glovsky and Popeo, P.C.
- Agent Muriel Liberto
- Priority: JP2014-233563 20141118
- Main IPC: C12Q1/6844
- IPC: C12Q1/6844 ; C12Q1/6853 ; C12Q1/686 ; C12N15/09 ; C12N9/00 ; C12N9/12 ; C12N9/16 ; C12N9/90 ; C12P19/34 ; C12N15/00
Abstract:
The present invention provides a method for easily and exponentially amplifying circular DNA, particularly long chain circular DNA, in a cell-free system. Specifically, the present invention provides a method for amplifying circular DNA in which circular DNA having a replication origin sequence (origin of chromosome (oriC)) is mixed with a reaction solution containing the following enzyme groups to form a reaction mixture, which is then reacted under an isothermal condition, the enzyme groups being:
(1) a first enzyme group that catalyzes replication of circular DNA;
(2) a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane and
(3) a third enzyme group that catalyzes a separation of two sister circular DNAs.
(1) a first enzyme group that catalyzes replication of circular DNA;
(2) a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane and
(3) a third enzyme group that catalyzes a separation of two sister circular DNAs.
Public/Granted literature
- US20190249236A1 METHOD OF AMPLIFYING CIRCULAR DNA Public/Granted day:2019-08-15
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